Application
PBX1 antibody immunoprecipitates PBX1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of PBX1 protein by 2 μg of PBX1 antibody 12 % SDS-PAGE The immunoprecipitated PBX1 protein was detected by PBX1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000| Product Name | PBX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PBX1 (pre-B-cell leukemia homeobox 1) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PBX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (100%), Rat (100%), Xenopus laevis (98%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686B09108 antibody MGC126627 antibody PBX1 antibody homeobox protein PRL antibody pre-B-cell leukemia transcription factor 1 antibody homeobox protein PBX1 antibody pre-B-cell leukemia homeobox 1 antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
PBX1 antibody immunoprecipitates PBX1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 μg of preimmune rabbit IgG B. Immunoprecipitation of PBX1 protein by 2 μg of PBX1 antibody 12 % SDS-PAGE The immunoprecipitated PBX1 protein was detected by PBX1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
