Application
PC1/3 antibody detects PC1/3 protein at cytosol and secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PC1/3 antibody dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PC1/3 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PC1/3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PC1/3 (proprotein convertase subtilisin/kexin type 1)The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PC1/3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Pig, BovineRat (100%), Zebrafish (93%), Pig (100%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BMIQ12 antibody NEC1 antibody PC1 antibody PC3 antibody SPC3 antibody PCSK1 antibody prohormone convertase 1 antibody prohormone convertase 3 antibody NEC 1 antibody neuroendocrine convertase 1 antibody proprotein convertase subtilisin/kexin type |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (3) |
| Cellular Localization | Cytoplasmic vesicle , secretory vesicle |
Application
PC1/3 antibody detects PC1/3 protein at cytosol and secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PC1/3 antibody dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PC1/3 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
