Application
GeneTex anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue).Application
Western blotting. Analysis shows the detection of human PC2 in probed lysates using GeneTex Affinity Purified anti-hPC2 antibody . The panel on the left shows the blot probed with anti-hPC2. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2. In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells.| Product Name | PC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PC2PC2 and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. |
| Immunogen | Synthetic peptide: GLQDSSTDNRAKLC, corresponding to amino acids 95 - 107 of Human PC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NBP16 CBX4 |
|---|---|
| Target | PC2 |
Application
GeneTex anti-hPC2 antibody was used for immunofluorescent imaging of human cells (U2OS). The image reveals the expected discrete nuclear structure that is termed the PcG body corresponding to the known localization of PC2. IF was performed after fixation in PBS with 4% PF for 5 min, permeabilization with 0.5% Polyethylene glycol tert-octylphenyl ether-PBS for 5 min, and blocking with 5% milk / 0.2% Tween for 1 h. Primary antibody used at 1:200 in 5% milk / 0.2% Tween for 1 h, secondary antibody for 30 min. All blocking and incubation steps carried out at 37° C. Nuclei were counterstained with Hoechst stain (blue).
Application
Western blotting. Analysis shows the detection of human PC2 in probed lysates using GeneTex Affinity Purified anti-hPC2 antibody . The panel on the left shows the blot probed with anti-hPC2. The panel on the right is the same blot reprobed with anti-FLAG antibody to confirm the presence of FLAG tagged recombinant PC2 in the lysate. In the left panel the band labeled as hPC2 is FLAG-tagged transfected hPC2 in 293T cells. The bands labeled with stars are likely endogenous hPC2. In the right panel the band labeled hPC2 is FLAG-tagged transfected hPC2 in 293T cells.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
