Application
Recombinant human PCAF was separated on SDS-PAGE, blotted with Anti-PCAF and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Negative control: without antibodyApplication
HeLa whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term diluted at 1:1000.Application
Lysate of Y79 cells was separated on SDS-PAGE, blotted with Anti-PCAF and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)Application
Rabbit Anti-PCAF was used to immunoprecipitate PCAF from an exctract of Jurkat cells. Detection antibody: Rabbit Anti-PCAF (C-terminal). Lanes 1. 5 μg/mL antibody 2. Negative control: without antibody 3. 10 μg/mL antibody 4. Negative control: without cell extractApplication
Nuclear extract of HeLa (lanes 1-2) and lysates of HepG2 (lanes 3-4) and Jurkat (lanes 5-6) cells were separated on SDS-PAGE, blotted with Anti-PCAF (817-832) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1,3,5: Antibody 2 μg/mL 2,4,6: Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)| Product Name | PCAF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PCAFCBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. |
| Immunogen | Synthetic peptide: EKFFFSKIKEAGLIDK (with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: 5-10μg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 10^7 human acute T cell leukemia Jurkat cells. WB: 1:500-1:300 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 88 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDog |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAF P P/CAF PCAF |
|---|---|
| Target | PCAF |
| Cellular Localization | Nuclear |
Application
Recombinant human PCAF was separated on SDS-PAGE, blotted with Anti-PCAF and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Negative control: without antibody
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term diluted at 1:1000.
Application
Lysate of Y79 cells was separated on SDS-PAGE, blotted with Anti-PCAF and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 2 μg/mL 2. Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)
Application
Rabbit Anti-PCAF was used to immunoprecipitate PCAF from an exctract of Jurkat cells. Detection antibody: Rabbit Anti-PCAF (C-terminal). Lanes 1. 5 μg/mL antibody 2. Negative control: without antibody 3. 10 μg/mL antibody 4. Negative control: without cell extract
Application
Nuclear extract of HeLa (lanes 1-2) and lysates of HepG2 (lanes 3-4) and Jurkat (lanes 5-6) cells were separated on SDS-PAGE, blotted with Anti-PCAF (817-832) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1,3,5: Antibody 2 μg/mL 2,4,6: Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)| Positive Control | Immunoblotting: Whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF.Immunoprecipitation: RIPA lysate, human acute T cell leukemia Jurkat cells |
|---|---|
| Application Notes | IP: 5-10μg of antibody immunoprecipitates PCAF from RIPA lysate with ~4 X 10^7 human acute T cell leukemia Jurkat cells. WB: 1:500-1:300 (determined using whole extracts of human epidermoid carcinoma A431, dog MDCK kidney cells or recombinant active human PCAF and a chemiluminescent detection reagent). Predicted molecular weight: 88 kDa. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
