Application
WB analysis of HepG2 cells using PCBP1 antibody at 1.25μg/ml. Lane A: marker Lane B: HepG2 cellsApplication
WB analysis of HepG2 cells using PCBP1 antibody at 2μg/ml. Lane A : Primary antibody Lane B : Primary antibody + Blocking peptide| Product Name | PCBP1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCBP1This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human PCBP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 93%; Dog: 92%; Guinea Pig: 93%; Horse: 93%; Human: 93%; Mouse: 93%; Rabbit: 93%; Rat: 93% |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | HNRNP X HNRNP E1 PCBP1 HNRPX HNRPE1 |
|---|---|
| Target | PCBP1 |
| Molecular Weight(MW) | 37 KDa (note) |
Application
WB analysis of HepG2 cells using PCBP1 antibody at 1.25μg/ml. Lane A: marker Lane B: HepG2 cells
Application
WB analysis of HepG2 cells using PCBP1 antibody at 2μg/ml. Lane A : Primary antibody Lane B : Primary antibody + Blocking peptide| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
