Application
Immunofluorescence analysis of methanol-fixed A431, using PCBP2 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000Application
PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PCBP2 antibody [N1C1] dilution: 1:500.Application
PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PCBP2 antibody [N1C1] dilution: 1:500.Application
Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody [N1C1] . Western blot analysis was performed using PCBP2 antibody [N1C1] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PCBP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCBP2 (poly(rC) binding protein 2)The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PCBP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, BovineRat (100%), Zebrafish (94%), Xenopus laevis (98%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HNRPE2 antibody MGC110998 antibody hnRNP-E2 antibody PCBP2 antibody heterogenous nuclear ribonucleoprotein E2 antibody heterogeneous nuclear ribonucleoprotein E2 antibody hnRNP E2 antibody poly(rC)-binding protein 2 antibody alpha-CP2 antibody pol |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (8) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed A431, using PCBP2 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000
Application
PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PCBP2 antibody [N1C1] dilution: 1:500.
Application
PCBP2 antibody [N1C1] detects PCBP2 protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PCBP2 antibody [N1C1] dilution: 1:500.
Application
Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody [N1C1] . Western blot analysis was performed using PCBP2 antibody [N1C1] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , Jurkat , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
