Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCBP3 antibody [N1C2] diluted at 1:1000.Application
Immunofluorescence analysis of methanol-fixed MCF-7, using PCBP3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000| Product Name | PCBP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCBP3 (poly(rC) binding protein 3)This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PCBP3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, BovineMouse (100%), Rat (94%), Zebrafish (92%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALPHA-CP3 antibody PCBP3 antibody poly(rC)-binding protein 3 antibody poly(rC) binding protein 3 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCBP3 antibody [N1C2] diluted at 1:1000.
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using PCBP3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
