Application
IHC-P analysis of human heart tissue using PCCA antibody at 1:600. Left to right:DAPI, PCCA, Merge. Heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.Application
WB analysis of tranfected 293T cells using PCCA antibody at 0.2-1μg/ml.| Product Name | PCCA antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCCAThe protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human PCCA |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 86%; Dog: 86%; Horse: 83%; Human: 100%; Mouse: 77%; Pig: 86%; Rabbit: 86%; Rat: 86%; Zebrafish: 75% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PCCA |
|---|---|
| Target | PCCA |
| Molecular Weight(MW) | 80 KDa (note) |
Application
IHC-P analysis of human heart tissue using PCCA antibody at 1:600. Left to right:DAPI, PCCA, Merge. Heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
Application
WB analysis of tranfected 293T cells using PCCA antibody at 0.2-1μg/ml.| Positive Control | Transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
