Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PCDHGB4 antibody at 1:500 dilution.| Product Name | PCDHGB4 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCDHGB4 (protocadherin gamma subfamily B, 4)This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. This particular family member is expressed in fibroblasts and is thought to play a role in wound healing in response to injury. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PCDHGB4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Chimpanzee, BovineMouse (85%), Chimpanzee (100%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDH20 antibody FIB2 antibody MGC138293 antibody MGC142167 antibody PCDH-GAMMA-B4 antibody PCDHGB4 antibody cadherin 20 antibody fibroblast cadherin-2 antibody fibroblast cadherin FIB2 antibody cadherin-20 antibody protocadherin gamma-B4 antibody |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (3) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PCDHGB4 antibody at 1:500 dilution.| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
