Application
PCK2 antibody [C1C2], Internal detects PCK2 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution.Application
PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PCK2 antibody [C1C2], Internal dilution: 1:1000Application
PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 7.5 % SDS-PAGE PCK2 antibody [C1C2], Internal dilution: 1:1000Application
PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE PCK2 antibody [C1C2], Internal dilution: 1:1000| Product Name | PCK2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCK2 (phosphoenolpyruvate carboxykinase 2 (mitochondrial))This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PCK2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PEPCK antibody PEPCK-M antibody PEPCK2 antibody PCK2 antibody phosphoenolpyruvate carboxylase antibody PEP carboxykinase antibody phosphopyruvate carboxylase antibody phosphoenolpyruvate carboxykinase [GTP] mitochondrial antibody phosphoenolpyruva |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (3) |
| Cellular Localization | Mitochondrion |
Application
PCK2 antibody [C1C2], Internal detects PCK2 protein at mitochondria by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PCK2 antibody at 1:100 dilution.
Application
PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PCK2 antibody [C1C2], Internal dilution: 1:1000
Application
PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract 7.5 % SDS-PAGE PCK2 antibody [C1C2], Internal dilution: 1:1000
Application
PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE PCK2 antibody [C1C2], Internal dilution: 1:1000| Positive Control | HepG2 , Neuro2A , GL261 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
