Application
Immunofluorescence analysis of methanol-fixed A431, using PCMT1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PCMT1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000| Product Name | PCMT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase)Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PCMT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PCMT1 antibody protein L-isoaspartyl/D-aspartyl methyltransferase antibody protein-beta-aspartate methyltransferase antibody protein-L-isoaspartate(D-aspartate) O-methyltransferase antibody L-isoaspartyl protein carboxyl methyltransferase antibody pro |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (6) |
Application
Immunofluorescence analysis of methanol-fixed A431, using PCMT1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PCMT1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
