Application
PCNA antibody detects PCNA protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody . Western blot analysis was performed using PCBP2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PCNA antibody dilution: 1:500.Application
PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody dilution: 1:500.Application
PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PCNA antibody dilution: 1:500.Application
PCNA antibody detects PCNA protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract 12% SDS-PAGE PCNA antibody dilution: 1:2500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PCNA antibody detects PCNA protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE PCNA antibody dilution: 1:2500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. PCNA antibody diluted at 1:500.Application
PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon . PCNA antibody diluted at 1:500.| Product Name | PCNA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineZebrafish (92%), Xenopus laevis (88%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC8367 antibody PCNA antibody cyclin antibody DNA polymerase delta auxiliary protein antibody proliferating cell nuclear antigen antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
PCNA antibody detects PCNA protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody diluted at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PCNA protein stained by PCNA antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody . Western blot analysis was performed using PCBP2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PCNA antibody dilution: 1:500.
Application
PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PCNA antibody dilution: 1:500.
Application
PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PCNA antibody dilution: 1:500.
Application
PCNA antibody detects PCNA protein by western blot analysis. A. 30 µg NIH-3T3 whole cell lysate/extract 12% SDS-PAGE PCNA antibody dilution: 1:2500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PCNA antibody detects PCNA protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE PCNA antibody dilution: 1:2500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. PCNA antibody diluted at 1:500.
Application
PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon . PCNA antibody diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12 , *A549 , *SiHa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
