Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.Application
ApplicationApplication
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 12% SDS PAGE diluted at 1:1000Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000| Product Name | PCNA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [339] to PCNA interdomain connector loopPCNA is a homotrimeric protein that is involved in DNA replication and DNA repair. It serves as a processivity factor.for DNA polymerase d. It has also been shown to interact with several other proteins that are involved in DNA replication and repair, including replication factor C, DNA ligase I, XPG, FEN1 and p21. This partiular antiserum was raised against the interdomain connector loop region of PCNA, which is the region of PCNA through which many of the protein-protein interactions occur. |
| Immunogen | Recombinant PCNA interdomain connector loop protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 339 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| For ELISA: Use at an assay dependent dilution. For WB: Use at a concentration of 1:500 - 1:1,000. Predicted molecular weight: 31.32 kDa. For ICC/IF: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC8367 |
|---|---|
| Target | PCNA |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
Application
Application
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 12% SDS PAGE diluted at 1:1000
Application
Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 12% SDS PAGE diluted at 1:1000| Application Notes | For ELISA: Use at an assay dependent dilution. For WB: Use at a concentration of 1:500 - 1:1,000. Predicted molecular weight: 31.32 kDa. For ICC/IF: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
