Application
WB analysis of indicated lysates using PCNA antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using PCNA antibody at a dilution of 1:100.Application
IP analysis of HeLa lysates using control IgG and PCNA antibody. The precipitates were detected by the same PCNA antibody.| Product Name | PCNA antibody [6B12-D7-H9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [6B12-D7-H9] to PCNAThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.[provided by RefSeq,Jul 2008] |
| Immunogen | Purified recombinant human PCNA protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 6B12-D7-H9 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| Species Reactivity | HumanMouseMonkeyRatHamster |
| Concentration | 1mg/ml |
| Gene Synonyms | proliferating cell nuclear antigen |
|---|---|
| Target | PCNA |
| Molecular Weight(MW) | 36kDa (note) |
| Cellular Localization | Nucleus. |
Application
WB analysis of indicated lysates using PCNA antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using PCNA antibody at a dilution of 1:100.
Application
IP analysis of HeLa lysates using control IgG and PCNA antibody. The precipitates were detected by the same PCNA antibody.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | ascites |
