Application
Human Tonsil stained with Anti-CD3, T Cell at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen at a 1:500 dilution (green). Counterstained with DAPI.Application
Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNAApplication
Staining of KM-H2 cells with MOUSE ANTI PCNA:FITC following permeabilisation| Product Name | PCNA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PC10] to PCNA-Proliferation MarkerPCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). |
| Immunogen | Protein A-rat PCNA (proliferating cell nuclear antigen) fusion obtained from pC2T |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | PC10 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-FIHC-PIPWB |
| Flow Cyt: Use at an assay dependent dilution. IHC-P: 1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol IHC-Fr: Use at an assay dependent dilution. Fix in 4% buffered formalin at room temperature for 2 min then fix in ethanol for 10 minutes. IP: Use at an assay dependent dilution WB: 1:5,000, Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRatFish |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | PCNA P12004 5111 176740 MGC8367 |
|---|---|
| Target | PCNA |
| Tissue Specificity | Specific for PCNA p36 protein expressed at high levels in proliferating cells |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Human Tonsil stained with Anti-CD3, T Cell at a 1:150 dilution (red) and Monoclonal Anti-Proliferating Cell Nuclear Antigen at a 1:500 dilution (green). Counterstained with DAPI.
Application
Western blot analysis of HeLa Nuclear Extract probed with Mouse anti Human PCNA
Application
Staining of KM-H2 cells with MOUSE ANTI PCNA:FITC following permeabilisation| Positive Control | WB: DT40 B lymphoma cell lysate, 293 cell lysate |
|---|---|
| Application Notes | Flow Cyt: Use at an assay dependent dilution. IHC-P: 1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol IHC-Fr: Use at an assay dependent dilution. Fix in 4% buffered formalin at room temperature for 2 min then fix in ethanol for 10 minutes. IP: Use at an assay dependent dilution WB: 1:5,000, Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline |
