| Product Name | PCOLCE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PCOLCEBMP1, also known as Bone Morphogenetic Protein 1, Procollagen C-endopeptidase, Procollagen C-peptidase, or Procollagen C-proteinase, is an extracellular zinc endopeptidase of the astacin family. PCOLE1 was described as an accessory protein that enhances the activity of BMP1. Another BMP1 enhancer protein, PCOLE2, was described in a subtractive library of eye tissue, and little is known about its function. PCOLE1 binds to Procollagen and assists BMP1, increasing the Type-I Procollagen C-Terminal processing activity 10-fold, although it does not directly activate the enzyme. BMP1 is produced in a number of different forms, with different C-terminal regions. PCOLE1 and 2 may be isoform- or tissuespecific enhancers, giving a degree of regulation to the activity of BMP1 in different circumstances. PCOLE1 is produced in tissues rich in Type-I collagen, such as skin, kidney and tendon. It is secreted into culture media by a wide range of cell types. |
| Immunogen | Synthetic peptide corresponding to the end of CUB2 and the beginning of the “linker” region of PCOLCE (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 of the antibody is recommended for chemiluminescent substrates. Detects a band of approximately 50-55 kDa. Proteolytic removal of the C-terminal end generates 34 and 36 kDa active fragments. Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanMousePig |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PCPE PCPE1 |
|---|---|
| Target | PCOLCE |
| Tissue Specificity | Does not react with PCOLE2 |
| Cellular Localization | Secreted |
| Application Notes | WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 of the antibody is recommended for chemiluminescent substrates. Detects a band of approximately 50-55 kDa. Proteolytic removal of the C-terminal end generates 34 and 36 kDa active fragments. Higher antibody dilutions may be necessary for non-human samples. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | F |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS |
