Application
Western blot of affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).Application
Human Breast (formalin-fixed, paraffin-embedded) stained with PDCD4 antibody at 1.25-2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Product Name | PDCD4 (phospho Ser457) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PDCD4 (phospho Ser457)This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Synthetic peptide |
| Modification | p-Ser457 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB (1:200 - 1:5000), ELISA (1:1500 - 1:7000), IHC-P (1.25 - 2.5 µg/ml): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PDCD4 (phospho S457) PDCD4 (phospho Ser457) PDCD4 (pS457) PDCD4 (pSer457) PDCD4 phospho S457 PDCD4 phospho Ser457 Phospho PDCD4 (S457) Phospho PDCD4 (Ser457) Phospho PDCD4 (pS457) Phospho PDCD4 (pSer457) |
|---|---|
| Target | PDCD4 |
| Tissue Specificity | Amino acids 447-465 of human Pdcd4 protein. |
Application
Western blot of affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).
Application
Human Breast (formalin-fixed, paraffin-embedded) stained with PDCD4 antibody at 1.25-2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.| Application Notes | WB (1:200 - 1:5000), ELISA (1:1500 - 1:7000), IHC-P (1.25 - 2.5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
