Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody diluted at 1:1000.| Product Name | PDCD4 (phospho Ser457) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDCD4 (phospho Ser457) (programmed cell death 4 (neoplastic transformation inhibitor))This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDCD4 (phospho Ser457). The exact sequence is proprietary. |
| Modification | p-Ser457 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineHuman (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | PDCD4 (phospho Ser457) |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 (phospho Ser457) antibody diluted at 1:1000.| Positive Control | NIH-3T3(starvation 16 hours and 5 ng/ml PDGF treatment for 24 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
