Application
PDCD4 antibody detects PDCD4 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 antibody diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PDCD4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | PDCD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDCD4 (programmed cell death 4 (neoplastic transformation inhibitor))This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDCD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chicken, Rhesus Monkey, BovineRat (96%), Chicken (87%), Rhesus Monkey (98%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H731 antibody MGC33046 antibody MGC33047 antibody PDCD4 antibody nuclear antigen H731 antibody protein 197/15a antibody neoplastic transformation inhibitor protein antibody programmed cell death protein 4 antibody programmed cell death 4 (neoplasti |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (3) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
PDCD4 antibody detects PDCD4 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PDCD4 antibody diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PDCD4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A431 , HeLa , Mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
