Application
Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | PDCD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [k4C1 ] to PDCD4Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells. |
| Immunogen | Recombinant human PDCD4 (1-469aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | k4C1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000~ 3,000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC33046 nuclear antigen H731 neoplastic transformation inhibitor Programmed cell death4 isoform1 MGC33047 H731 PDPC4 Programmed cell death 4 isoform 1 programmed cell death4 (neoplastic transformation inhibitor) PDCD4 programmed cell death 4 (ne |
|---|---|
| Target | PDCD4 |
| Cellular Localization | Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. |
| Light Chain Type | kappa |
Application
Cell lysates of HepG2(40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD4 (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:1,000~ 3,000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
