Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PDCD6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE diluted at 1:500Application
PDCD6 antibody detects PDCD6 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. PDCD6 antibody diluted at 1:500.| Product Name | PDCD6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDCD6 (programmed cell death 6)This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDCD6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (99%), Rat (98%), Zebrafish (88%), Xenopus laevis (86%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALG-2 antibody FLJ46208 antibody MGC111017 antibody MGC119050 antibody MGC9123 antibody PEF1B antibody PDCD6 antibody apoptosis-linked gene-2 antibody apoptosis-linked gene 2 protein antibody programmed cell death protein 6 antibody probable calc |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Nucleus membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein , Nucleus |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PDCD6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE diluted at 1:500
Application
PDCD6 antibody detects PDCD6 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. PDCD6 antibody diluted at 1:500.| Positive Control | HeLa , HeLa nucleus |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
