Application
Western blot analysis of PDE1C -1,2,3 using Lane 1: Brain lysate Lane 2: Heart lysate Lane 3: Lung lysate Lane 4: Testis lysate Lane 5: Kidney lysateApplication
Western blot of PDE1C with samplesApplication
IHC of artery walls with antibody| Product Name | PDE1C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE1CCyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodiesterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1C gene has been cloned from human, mouse and rat. The PDE1C gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. The tissue distribution of PDE1C is different from other PDE1 gene family members. A 5 splice variant of PDE1C2 is found in rat olfactory epithelium and is believed to be involved in olfactory signal transduction. Other PDE1C variants (PDE1C1 and PDE1C2) are found in human heart. The PDE1C enzymes bind both cGMP and cAMP with different affinities. |
| Immunogen | Synthetic peptide (N terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| For ICC: Use at a dilution of 1:200. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/250 - 1/500. This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Hcam3 5137 602987 Q14123 PDE1C |
|---|---|
| Target | PDE1C |
| Tissue Specificity | The antibody does not cross react with PDE1A, PDE1B or other PDEs. |
Application
Western blot analysis of PDE1C -1,2,3 using Lane 1: Brain lysate Lane 2: Heart lysate Lane 3: Lung lysate Lane 4: Testis lysate Lane 5: Kidney lysate
Application
Western blot of PDE1C with samples
Application
IHC of artery walls with antibody| Application Notes | For ICC: Use at a dilution of 1:200. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/250 - 1/500. This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
