Application
WB using to detect increased phosphorylation of PDE4A6 in short-term FSK stimulation compared to the unchanged amount of PDE4A6 at 87 kDa.Application
WB of using PDE4A5 with and without PKA-dependent phosphorylation. 1:500 antibody dilution in DiluObuffer.Application
WB of and with phosphorylated and non phosphorylated PDE4D protein. 1:500 antibody dilution in DiluObuffer| Product Name | PDE4A (phospho Ser140) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDE4A (phospho Ser140)The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family | and PDE4 subfamily. This PDE hydrolyzes the second messenger | cAMP | which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus | by regulating the cellular concentration of cAMP | this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq | Jul 2011] |
| Immunogen | Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine 140 residue in the rat/mouse PDE4A5 variant. This site is conserved in other PDE4A long form variants. |
| Modification | p-Ser140 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| WB: 1:500. IP: 1:150. ELISA: 1:10000 | |
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DPDE2 , PDE46 , phosphodiesterase 4A , PDE4A , PDE4 |
|---|---|
| Target | PDE4A (phospho Ser140) |
| Tissue Specificity | This antibody detects all known long-form variants of PDE4A | including PDE4A4 | PDE4A5 | PDE4A8 | PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with other members of the PDE family including PDE4B or PDE4D. |
Application
WB using to detect increased phosphorylation of PDE4A6 in short-term FSK stimulation compared to the unchanged amount of PDE4A6 at 87 kDa.
Application
WB of using PDE4A5 with and without PKA-dependent phosphorylation. 1:500 antibody dilution in DiluObuffer.
Application
WB of and with phosphorylated and non phosphorylated PDE4D protein. 1:500 antibody dilution in DiluObuffer| Application Notes | WB: 1:500. IP: 1:150. ELISA: 1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20⁰C for long term storage |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
