Application
Western blot of variant-selective antibody. Antibody dilution 1:500| Product Name | PDE4D3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE4D3Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins. |
| Immunogen | Synthetic peptide from unique region near N-terminal of the PDE4D3 variant. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at an assay dependent dilution. IP: Use at a dilution of 1/150. WB: Use at a dilution of 1/500. Detects a band of approximately 93 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Phosphodiesterase Type 4D3 |
|---|---|
| Target | PDE4D3 |
| Tissue Specificity | does not cross react with PDE4D1, D2, D4 and D5. Does not cross react with PDE4A, PDE4B or PDE4C. |
Application
Western blot of variant-selective antibody. Antibody dilution 1:500| Positive Control | Rat cortical membranes |
|---|---|
| Application Notes | ICC: Use at an assay dependent dilution. IP: Use at a dilution of 1/150. WB: Use at a dilution of 1/500. Detects a band of approximately 93 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide |
