| Product Name | PDE4D5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE4D5Cyclic AMP-dependent Phosphodiesterase 4 type D (PDE4D) family includes 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation and selective phosphorylation by PKA and other kinases. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanisms involved protein-protein interactions with cytoskeletal scaffolding proteins. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (N terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/200 - 1/250. Detects a band of approximately 105 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cAMP-specific 3'5'-cyclic phosphodiesterase 4D dunce-like phosphodiesterase E3 Phosphodiesterase 4D cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) |
|---|---|
| Target | PDE4D5 |
| Tissue Specificity | This antibody does not cross-react with other members of the PDE4D family or with other PDE4 proteins (PDE4A, B and C) or with other PDE family members. |
| Positive Control | Rat cortical membranes |
|---|---|
| Application Notes | WB: Use at a dilution of 1/200 - 1/250. Detects a band of approximately 105 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide |
