Application
Immunostaining of cells expressing PDE5 with antibodyApplication
Western blot of human recombinant GFP-PDE5A with PDE5A and Phospho-PDE5A antibodies. Overexpressing cells were treated with or with out ANP. Antibody dilution 1:500 in DiluObuffer. The blot was stripped with StripObuffer and re-probed with Phospho-PDE5A antibody| Product Name | PDE5A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE5AThe cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB PDEs). The cGB PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. |
| Immunogen | Synthetic cyclic peptide (common to all PDE5A variants) (Rat). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects bands of approximately 99 and 89 kDa. (predicted molecular weight: 105 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PDE5A2 |
|---|---|
| Target | PDE5A |
| Tissue Specificity | This antibody detects 99 and 89 kDa PDE5A proteins in various tissues. |
| Cellular Localization | Located mostly to soluble cellular fractions, and sometimes to particulate cellular fractions. |
Application
Immunostaining of cells expressing PDE5 with antibody
Application
Western blot of human recombinant GFP-PDE5A with PDE5A and Phospho-PDE5A antibodies. Overexpressing cells were treated with or with out ANP. Antibody dilution 1:500 in DiluObuffer. The blot was stripped with StripObuffer and re-probed with Phospho-PDE5A antibody| Positive Control | Rat lung homogenate |
|---|---|
| Application Notes | IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects bands of approximately 99 and 89 kDa. (predicted molecular weight: 105 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide |
