Application
PDE8A antibody detects PDE8A protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: PDE8A protein stained by PDE8A antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | PDE8A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDE8A (phosphodiesterase 8A)Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP. These cyclic nucleotides play an important role as second messengers in multiple physiologic processes, including regulation of vascular resistance, cardiac output, visceral motility, immune response, inflammation, neuroplasticity, vision, and reproduction. PDEs comprise a large superfamily of enzymes divided into 10 families. Different PDEs can be distinguished by their structure, tissue expression, localization, substrate specificity, regulation, and sensitivity to PDE inhibitors. Diversity in structure and specificity of function make PDEs promising targets for the pharmacotherapy of diseases modulated by cyclic nucleotide signaling (Hetman et al., MIM 2000). See MIM 171885.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDE8A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rhesus Monkey, BovineMouse (80%), Rhesus Monkey (99%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HsT19550 phosphodiesterase 8A |
|---|---|
| Target | PDE8A |
| Molecular Weight(MW) | 93 kDa (note) |
Application
PDE8A antibody detects PDE8A protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: PDE8A protein stained by PDE8A antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
