Application
Western blot using antibody| Product Name | PDE8A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDE8AOne of the many phosphodiesterases that compartmentalized and hydrolyze cAMP into AMP is phosphodiesterase type 8. The cAMP-specific phosphodiesterase type 8 (PDE8) family is comprised of 2 genes (PDE8A and PDE8B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE8 family is a high affinity cAMP-specific, IBMX sensitive PDE. The PDE8 has a significant conserved region of about 270 amino acids common to all PDEs at the carboxy terminal apparently serves as the catalytic domain. The amino-terminal region of this protein is divergent and presumably accounts for the distinctive and regulatory properties unique to the individual PDE families. PDE8A protein showed significant homology to other cAMP-dependent PDEs (23%) within the catalytic domain. PDE8A is widely expressed in various tissues in contrast to PDE8B that is exclusively expressed in thyroid gland. The PDE8A transcripts are found in brain, pancreas, placenta, thyroid, spleen, trachea, prostate, and uterus. |
| Immunogen | Synthetic cyclic peptide, corresponding to the mid region of PDE8A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-102 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | cAMP-specific cyclic nucleotide phosphodiesterase 8A High-affinity cAMP-specific and IBMX-insensitive 3'5'-cyclic phosphodiesterase 8A HsT19550 Phosphodiesterase 8A Weakly similar to 3'5'-cyclic-nucleotide phosphodiesterase |
|---|---|
| Target | PDE8A |
Application
Western blot using antibody| Application Notes | IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 98-102 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
