Application
Sample (30 ug of whole cell lysate) A: K562 5% SDS PAGE diluted at 1:1000Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal diluted at 1:500.| Product Name | PDGF Receptor alpha antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PDGF Receptor alpha(CD140a) (platelet-derived growth factor receptor, alpha polypeptide)This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDGF Receptor alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, BovineXenopus laevis (82%), Chicken (86%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD140A antibody MGC74795 antibody PDGFR2 antibody RHEPDGFRA antibody PDGFRA antibody CD140 antigen-like family member A antibody CD140a antigen antibody PDGF-R-alpha antibody rearranged-in-hypereosinophilia-platelet derived growth factor receptor a |
|---|---|
| Molecular Weight(MW) | 123 kDa (note) (2) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample (30 ug of whole cell lysate) A: K562 5% SDS PAGE diluted at 1:1000
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PDGF Receptor alpha antibody [N2C2], Internal diluted at 1:500.| Positive Control | K562 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
