Application
Sample(30 µg whole cell lysate) A:HeLa S3 B:Hep G2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A431, using PDGF Receptor beta antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PDGF Receptor beta(CD140b) antibody at 1:500 dilution.Application
PDGF Receptor beta antibody detects PDGF Receptor beta protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with PDGF Receptor beta antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PDGF Receptor beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDGF Receptor beta (platelet-derived growth factor receptor, beta polypeptide)This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PDGF Receptor beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Dog, BovineDog (82%), Bovine (80%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD140B antibody JTK12 antibody PDGFR antibody PDGFR1 antibody PDGFRB antibody PDGFR-beta antibody platelet-derived growth factor receptor 1 antibody PDGF-R-beta antibody platelet-derived growth factor receptor beta antibody beta-type platelet-deri |
|---|---|
| Molecular Weight(MW) | 124 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Sample(30 µg whole cell lysate) A:HeLa S3 B:Hep G2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A431, using PDGF Receptor beta antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using PDGF Receptor beta(CD140b) antibody at 1:500 dilution.
Application
PDGF Receptor beta antibody detects PDGF Receptor beta protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 5% SDS-PAGE, and blotted with PDGF Receptor beta antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLaS3 , HepG2 , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
