Application
Flow cytometric analysis of SK-Br-3 cells using PDGFRB Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PDGFRB Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.Application
Western blot analysis of anti-PDGFRB Antibody (N-term) in cell line lysates (35ug/lane). PDGFRB (arrow) was detected using the purified Pab.| Product Name | PDGF Receptor beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDGF Receptor betaProtein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families). |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 54-72 of Human PDGF RB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| For ELISA: Use at a dilution of 1:900 - 1:1,100. For IHC-F and IHC-P: Use at a dilution of 1:50 - 1:100. For WB: Use at a dilution of 1:100 - 1:500. Detects a band of approximately 122 kDa (predicted molecular weight: 132 kDa). Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | CD140B JTK12 PDGFR PDGFR1 |
|---|---|
| Target | PDGFR beta |
| Cellular Localization | Cell Membrane and Cytoplasmic. |
Application
Flow cytometric analysis of SK-Br-3 cells using PDGFRB Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PDGFRB Antibody (N-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Application
Western blot analysis of anti-PDGFRB Antibody (N-term) in cell line lysates (35ug/lane). PDGFRB (arrow) was detected using the purified Pab.| Positive Control | THP-1 cell lysate |
|---|---|
| Application Notes | For ELISA: Use at a dilution of 1:900 - 1:1,100. For IHC-F and IHC-P: Use at a dilution of 1:50 - 1:100. For WB: Use at a dilution of 1:100 - 1:500. Detects a band of approximately 122 kDa (predicted molecular weight: 132 kDa). Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline containing 0.09% sodium azide |
