Application
Western blot analysis of extracts of various cell lines, using P4HB antibody.Application
Immunofluorescence analysis of HeLa cell using P4HB antibody. Blue: DAPI for nuclear staining.| Product Name | PDI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDIThis gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human P4HB |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPRIPWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100, IP: 1:20 - 1:50, RIP: 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P4Hbeta DSI prolyl 4-hydroxylase subunit beta PO4HB PHDB PROHB PO4DB ERBA2L P4HB GIT PDIA1 |
|---|---|
| Target | PDI |
Application
Western blot analysis of extracts of various cell lines, using P4HB antibody.
Application
Immunofluorescence analysis of HeLa cell using P4HB antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20 - 1:100, IP: 1:20 - 1:50, RIP: 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
