Application
Flow cytometry analysis of PDI in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with PDI antibody [RL90] at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.Application
Western blot analysis of PDI in 25 ug of HepG2, HeLa and NIH-3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with PDI antibody [RL90] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.Application
Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with PDI antibody [RL90] at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with a proper secondary antibody. Actin was stained with Dylight 350 Phalloidin and nuclei (red) were stained with DRAQ5. Images were taken at 20X magnification.| Product Name | PDI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [RL90] to PDImay be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010] |
| Immunogen | Purified rat PDI protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | RL90 |
| Host Species | Mouse |
| Tested Applications | BlockingELISAFACSICC/IFIHCIHC-FIHC-PIMIPWB |
| ICC/IF: 1:100 - 1:1000: |
|
| Species Reactivity | HumanMousePigRatHamsterPrimateRabbitBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | prolyl 4-hydroxylase beta polypeptide P4hb PDIR |
|---|---|
| Target | PDI |
Application
Flow cytometry analysis of PDI in HeLa cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with PDI antibody [RL90] at a dilution of 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.
Application
Western blot analysis of PDI in 25 ug of HepG2, HeLa and NIH-3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with PDI antibody [RL90] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
Application
Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with PDI antibody [RL90] at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with a proper secondary antibody. Actin was stained with Dylight 350 Phalloidin and nuclei (red) were stained with DRAQ5. Images were taken at 20X magnification.| Application Notes | ICC/IF: 1:100 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Contains 0.05 % sodium azide and 1 mg/ml BSA |
