Application
Confocal immunofluorescent analysis of PDK4 Antibody (Center D98) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center D98) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-PDK4 Antibody (Center D98) in HL60 cell line lysates (35ug/lane).PDK4(arrow) was detected using the purified Pab.| Product Name | PDK4 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PDK4 (Internal D98)Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. |
| Immunogen | KLH conjugated synthetic peptide between 90~119 amino acids from the center(D98) region of human PDK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Target | PDK4 |
|---|---|
| Molecular Weight(MW) | 46.469 kDa (note) |
Application
Confocal immunofluorescent analysis of PDK4 Antibody (Center D98) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 antibody (Center D98) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-PDK4 Antibody (Center D98) in HL60 cell line lysates (35ug/lane).PDK4(arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
