Application
Sample (50 ug of whole cell lysate) A: mouse muscle 10% SDS PAGE diluted at 1:1000Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PDLIM3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDLIM3 (PDZ and LIM domain 3)The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDLIM3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Chicken, Rhesus Monkey, BovineHuman (100%), Chicken (84%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALP antibody DKFZp686L0362 antibody FLJ26096 antibody PDLIM3 antibody actinin-associated LIM protein antibody enigma homolog antibody alpha-actinin-2-associated LIM protein antibody PDZ and LIM domain protein 3 antibody PDZ and LIM domain 3 antibod |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Cytoplasm , myofibril , sarcomere , Z-disk |
Application
Sample (50 ug of whole cell lysate) A: mouse muscle 10% SDS PAGE diluted at 1:1000
Application
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PDLIM3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | mouse muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
