Application
Western Blot analysis of PDLIM3 expression in transfected 293T cell line by PDLIM3 polyclonal antibody. A: Non-transfected lysate. B: PDLIM3 transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | PDLIM3 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PDLIM3 (PDZ and LIM domain 3)The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PDLIM3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ALP antibody DKFZp686L0362 antibody FLJ26096 antibody PDLIM3 antibody actinin-associated LIM protein antibody enigma homolog antibody alpha-actinin-2-associated LIM protein antibody PDZ and LIM domain protein 3 antibody PDZ and LIM domain 3 antibod |
|---|---|
| Target | PDLIM3 |
| Molecular Weight(MW) | 39 kDa (note) (4) |
| Cellular Localization | Cytoplasm , myofibril , sarcomere , Z-disk |
Application
Western Blot analysis of PDLIM3 expression in transfected 293T cell line by PDLIM3 polyclonal antibody. A: Non-transfected lysate. B: PDLIM3 transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | PDLIM3 tansfected 293T cell |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
