Application
Immunofluorescent staining of HT29 cells using anti-PDX1 mouse monoclonal antibody .Application
Anti-PDX1 mouse monoclonal antibody immunofluorescent staining of HELA cells transiently transfected with PDX1Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1.| Product Name | Pdx1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2A12] to Pdx1Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-283 of human Pdx1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2A12 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | IUF1 IDX1 STF-1 IDX-1 STF1 MODY4 PDX-1 PDX1 STF 1 IDX 1 PDX 1 |
|---|---|
| Target | Pdx1 |
| Tissue Specificity | is specific for amino acids 1-283 of human Pdx1 |
Application
Immunofluorescent staining of HT29 cells using anti-PDX1 mouse monoclonal antibody .
Application
Anti-PDX1 mouse monoclonal antibody immunofluorescent staining of HELA cells transiently transfected with PDX1
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PDX1 mouse monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
