Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using PDZK1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Product Name | PDZK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDZK1 (PDZ domain containing 1)A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDZK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rabbit, BovineRabbit (86%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAP70 antibody CLAMP antibody NHERF-3 antibody NHERF3 antibody PDZD1 antibody PDZK1 antibody CFTR-associated protein of 70 kDa antibody Na(+)/H(+) exchange regulatory cofactor NHE-RF3 antibody sodium-hydrogen exchanger regulatory factor 3 antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (4) |
| Cellular Localization | Cytoplasm (By similarity) , Membrane; Peripheral membrane protein (By similarity) |
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using PDZK1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
