| Product Name | PEDF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PEDFPigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human Y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts. It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina. PEDF is a potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The PEDF gene is a member of the serpin gene family. Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity. |
| Immunogen | His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). 40μg of Y79 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti PEDF (1μg/ml). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | 5176 P36955 EPC-1 172860 EPC1 SERPINF1 PIG35 PEDF EPC 1 |
|---|---|
| Target | PEDF |
| Tissue Specificity | Recognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms. |
| Cellular Localization | Secreted |
| Positive Control | RIPA lysates from Y79 cells |
|---|---|
| Application Notes | WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). 40μg of Y79 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti PEDF (1μg/ml). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: Supplied as a liquid in 0.1M Tris glycine, 0.15M sodium chloride, before the addition of glycerol to 30%. pH 7.4 |
