Application
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1 antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin antibody at 1:200 dilution.Application
Sample(30 ug whole cell lysate) A:293T 12% SDS PAGE diluted at 1:1000| Product Name | PEF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PEF1 (penta-EF-hand domain containing 1)PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1; MIM 114170), sorcin (SRI; MIM 182520), grancalcin (GCA; MIM 607030), and ALG2 (PDCD6; MIM 601057) (Kitaura et al., 2001 [PubMed 11278427]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PEF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (92%), Rat (91%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ABP32 antibody PEF1A antibody PEF1 antibody peflin antibody PEF protein with a long N-terminal hydrophobic domain antibody penta-EF hand domain containing 1 antibody penta-EF-hand domain containing 1 antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) |
| Cellular Localization | Cytoplasm , Membrane; Peripheral membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using PEF1 antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Peflin antibody at 1:200 dilution.
Application
Sample(30 ug whole cell lysate) A:293T 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
