Application
Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.Application
Flow cytometric Analysis of Hela cells, using anti-PEPD antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Product Name | PEPD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5D5] to PEPDSplits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. |
| Immunogen | Full length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5D5 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| Flow Cytometry 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PEPD MGC10905 PROLIDASE |
|---|---|
| Target | PEPD |
| Tissue Specificity | is specific for human PEPD (NP_000276). |
Application
Flow cytometric Analysis of Jurkat cells, using anti-PEPD antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
Application
Flow cytometric Analysis of Hela cells, using anti-PEPD antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).| Application Notes | Flow Cytometry 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
