Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of PERK Antibody (N-term Q163) in 293 cell line lysates (35ug/lane). PERK (arrow) was detected using the purified Pab.| Product Name | PERK antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PERK (N-term Q163)The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 154~184 amino acids from the N-term region of human PERK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Pancreatic eIF2 alpha kinase Pancreatic eIF2alpha kinase |
|---|---|
| Target | PERK |
| Molecular Weight(MW) | 125.146 kDa (note) |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of PERK Antibody (N-term Q163) in 293 cell line lysates (35ug/lane). PERK (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
