Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PEX19 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample (30 µg of whole cell lysate) A: Raji B: K562 C: THP-1 D: NCI-H929 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PEX19 antibody detects PEX19 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 10% SDS-PAGE PEX19 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PEX19 antibody detects PEX19 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PEX19 antibody dilution: 1:500.| Product Name | PEX19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PEX19 (peroxisomal biogenesis factor 19)This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. The peroxisomal biogenesis disorders are a heterogeneous group with at least 14 complementation groups and with more than 1 phenotype being observed in cases falling into particular complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause Zellweger syndrome (ZWS). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PEX19. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | D1S2223E antibody FLJ55296 antibody HK33 antibody PMP1 antibody PMPI antibody PXF antibody PXMP1 antibody PEX19 antibody peroxisomal biogenesis factor 19 antibody peroxisomal farnesylated protein antibody housekeeping gene 33kD antibody 33 kDa |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Cytoplasm , Peroxisome membrane; Lipid-anchor; Cytoplasmic side |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PEX19 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample (30 µg of whole cell lysate) A: Raji B: K562 C: THP-1 D: NCI-H929 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PEX19 antibody detects PEX19 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 10% SDS-PAGE PEX19 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PEX19 antibody detects PEX19 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PEX19 antibody dilution: 1:500.| Positive Control | Raji , K562 , THP-1 , NCI-H929 , mouse liver , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
