Application
HEK293T cells transfected with either RC202062 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PEX5 antibody, and then analyzed by flow cytometry.Application
Anti-PEX5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PEX5Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEX5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEX5.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PEX5 monoclonal antibody.| Product Name | PEX5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [6E9] to PEX5Binds to the C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type) and plays an essential role in peroxisomal protein import. |
| Immunogen | Full length human recombinant protein of human PEX5 (NP_000310) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6E9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PXR1 PTS1R PEX5 |
|---|---|
| Target | PEX5 |
| Tissue Specificity | is specific for human PEX5 (NP_000310). |
| Cellular Localization | Its distribution appears to be dynamic. It is probably a cycling receptor found mainly in the cytoplasm and as well associated to the peroxisomal membrane through a docking factor. |
Application
HEK293T cells transfected with either RC202062 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PEX5 antibody, and then analyzed by flow cytometry.
Application
Anti-PEX5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PEX5
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEX5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEX5.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PEX5 monoclonal antibody.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
