Application
PFKFB3 antibody [N1N3] detects PFKFB3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PFKFB3 protein stained by PFKFB3 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000| Product Name | PFKFB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3)Synthesis and degradation of fructose 2,6-bisphosphate. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PFKFB3. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Mouse, Rat, BovineMouse (97%), Rat (97%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ37326 antibody IPFK2 antibody PFK2 antibody PFKFB3 antibody PFK/FBPase 3 antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (6) |
Application
PFKFB3 antibody [N1N3] detects PFKFB3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PFKFB3 protein stained by PFKFB3 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
