Application
PGAM2 antibody detects PGAM2 protein by Western blot analysis. A. 50 µg rat muscle lysate/extract 12 % SDS-PAGE PGAM2 antibody dilution: 1:10000Application
PGAM2 antibody detects PGAM2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGAM2 protein stained by PGAM2 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 12% SDS PAGE diluted at 1:10000| Product Name | PGAM2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PGAM2 (phosphoglycerate mutase 2 (muscle))Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. |
| Immunogen | Full length human PGAM2 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Pig, BovineMouse (92%), Zebrafish (86%), Xenopus laevis (84%), Pig (96%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSD10 antibody MGC88743 antibody PGAM-M antibody PGAMM antibody PGAM2 antibody phosphoglycerate mutase isozyme M antibody BPG-dependent PGAM 2 antibody phosphoglycerate mutase 2 antibody muscle-specific phosphoglycerate mutase antibody phosphoglyc |
|---|---|
| Target | PGAM2 |
| Molecular Weight(MW) | 29 kDa (note) |
Application
PGAM2 antibody detects PGAM2 protein by Western blot analysis. A. 50 µg rat muscle lysate/extract 12 % SDS-PAGE PGAM2 antibody dilution: 1:10000
Application
PGAM2 antibody detects PGAM2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGAM2 protein stained by PGAM2 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: Hela 12% SDS PAGE diluted at 1:10000| Positive Control | H1299 , HeLa , rat muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
