Application
Western blot analysis of PPARGC1A in human heart tissue lysate with PPARGC1A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of PPARGC1A in rat heart tissue with PPARGC1A antibody at 5 ug/mL.Application
Immunofluorescence of PPARGC1A in rat heart tissue with PPARGC1A antibody at 20 ug/mL.| Product Name | PGC1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PGC1 alphaThe protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008] |
| Immunogen | PPARGC1A antibody was raised against a 17 amino acid peptide near the amino terminus of human PPARGC1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB:1:500~1:1000 IP:1:100~1:500 IF:1:100~1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PGC1A PPARGC1A LEM6 PGC1 PPARGC1 PGC-1(alpha) PGC-1v peroxisome proliferator-activated receptor gamma coactivator 1 alpha |
|---|---|
| Target | PGC1 alpha |
| Tissue Specificity | PPARGC1A antibody is human and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms. |
Application
Western blot analysis of PPARGC1A in human heart tissue lysate with PPARGC1A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of PPARGC1A in rat heart tissue with PPARGC1A antibody at 5 ug/mL.
Application
Immunofluorescence of PPARGC1A in rat heart tissue with PPARGC1A antibody at 20 ug/mL.| Application Notes | WB:1:500~1:1000 IP:1:100~1:500 IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
