Application
Immunohistochemical staining of PHAP III in human small intestine with PHAP III antibody at 2μg/ml.Application
Western blot analysis of PHAP III in A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP III antibody at 1μg/ml. Positive control: A549 cell lysate.| Product Name | PHAP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PHAP3Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation. |
| Immunogen | Synthetic peptide (Human). (NP_112182) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: Use at a concentration of 0.5 - 2.0 μg/ml. Detects a band of approximately 35 kDa. Can be blocked with GTX26242 PHAP3 peptide (human) Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | PHAP3 |
|---|---|
| Tissue Specificity | This antibody does not cross-react with PHAPI or PHAP12a. |
Application
Immunohistochemical staining of PHAP III in human small intestine with PHAP III antibody at 2μg/ml.
Application
Western blot analysis of PHAP III in A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP III antibody at 1μg/ml. Positive control: A549 cell lysate.| Positive Control | A549 or HepG2 whole cell lysate |
|---|---|
| Application Notes | WB: Use at a concentration of 0.5 - 2.0 μg/ml. Detects a band of approximately 35 kDa. Can be blocked with GTX26242 PHAP3 peptide (human) Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
