Application
Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is treated with the synthesized peptide.Application
Western blot analysis of extracts from COLO cells, using PHCA Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | PHCA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PHCAHydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PHCA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EC3.5.1. Alkaline phytoceramidase Alkaline dihydroceramidase SB89 aPHC Alkaline ceramidase EC 3.5.1. PHCA EC 3.5.1.- |
|---|---|
| Target | PHCA |
| Molecular Weight(MW) | 31 kDa (note) |
| Tissue Specificity | PHCA Antibody detects endogenous levels of total PHCA protein. |
Application
Immunofluorescence analysis of MCF7 cells, using PHCA Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using PHCA Antibody. The picture on the right is treated with the synthesized peptide.
Application
Western blot analysis of extracts from COLO cells, using PHCA Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
