Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PHD4 antibody at 1:500 dilution.| Product Name | PHD4 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIF Prolyl Hydroxylase 4 (prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum))The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PHD4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (94%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EGLN4 antibody HIFPH4 antibody P4H-TM antibody PH-4 antibody PH4 antibody PHD4 antibody P4HTM antibody hypoxia inducible factor prolyl 4 hydroxylase antibody proline 4-hydroxylase antibody hypoxia-inducible factor prolyl hydroxylase 4 antibody HI |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PHD4 antibody at 1:500 dilution.| Positive Control | A549 , H1299 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
